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EYA4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EYA4 Antibody (C-term) AP9330b
    Western blot analysis of EYA4 Antibody (C-term) (Cat. #AP9330b) in A2058 cell line lysates (35ug/lane). EYA4 (arrow) was detected using the purified Pab.
  • IHC-P - EYA4 Antibody (C-term) AP9330b
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with EYA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - EYA4 Antibody (C-term) AP9330b
    EYA4 Antibody (C-term) (Cat. #AP9330b) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95677
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 69505 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 2070
Other Names Eyes absent homolog 4, EYA4
Target/Specificity This EYA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the C-terminal region of human EYA4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEYA4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EYA4
Function Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye (By similarity).
Cellular Location Cytoplasm. Nucleus
Tissue Location Highly expressed in heart and skeletal muscle.
Research Areas
Citations (0)

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EYA4 encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti.


Miller,S.J. Oncogene 29 (3), 368-379 (2010)
Li,H. J. Exp. Clin. Cancer Res. 28, 145 (2009)
Melzer,D. PLoS Genet. 4 (5), E1000072 (2008)

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$ 295.00
$ 99.00
Cat# AP9330b
(40 western blots)
Availability: In Stock
Bulk Size
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