|Application ||WB, IHC-P, FC, E|
|Calculated MW||34324 Da|
|Antigen Region||126-155 aa|
|Other Names||Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid dehydrogenase 12, 17-beta-HSD 12, 3-ketoacyl-CoA reductase, KAR, 131-, HSD17B12|
|Target/Specificity||This HSD17B12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the Central region of human HSD17B12.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HSD17B12 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the second of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3- ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The enzyme 17-beta hydroxysteroid dehydrogenase-12 (HSD17B12) uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation.
Plourde,M. J. Steroid Biochem. Mol. Biol. 116 (3-5), 134-153 (2009)
Nagasaki,S. Mol. Cell. Endocrinol. 307 (1-2), 163-168 (2009)
Bellemare,V. J. Steroid Biochem. Mol. Biol. 114 (3-5), 129-134 (2009)
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