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TREF1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, FC, E |
|---|---|
| Primary Accession | Q96PN7 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 132256 Da |
| Antigen Region | 1136-1164 aa |
| Gene ID | 55809 |
|---|---|
| Other Names | Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132, TRERF1, BCAR2, RAPA, TREP132 |
| Target/Specificity | This TREF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1136-1164 amino acids from the C-terminal region of human TREF1. |
| Dilution | IF~~1:10~50 WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TREF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRERF1 |
|---|---|
| Synonyms | BCAR2, RAPA, TREP132 |
| Function | Binds DNA and activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1. |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00512, ECO:0000255|PROSITE-ProRule:PRU00624, ECO:0000269|PubMed:11349124, ECO:0000269|PubMed:16371131} |
| Tissue Location | Highest expression was seen in thymus, testis and adrenal cortex, expressed also in the adrenal medulla, thyroid, and stomach. Highly expressed in steroidogenic JEG-3 and MCF-7 cells, low expression was seen in non-steroidogenic Hep-G2 and HEK293 cells |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
TREF1 encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1.
References
Gizard,F. Mol. Cell. Biol. 26 (20), 7632-7644 (2006)
Gizard,F. Mol. Cell. Biol. 25 (11), 4335-4348 (2005)
Beausoleil,S.A. Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135 (2004)
Gizard,F. J. Mol. Endocrinol. 32 (2), 557-569 (2004)
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