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T150A Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - T150A Antibody (Center) AP9366c
    Western blot analysis of T150A Antibody (Center) (Cat. #AP9366c) in Jurkat cell line lysates (35ug/lane). T150A (arrow) was detected using the purified Pab.
    detail
  • IHC-P - T150A Antibody (Center) AP9366c
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with T150A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - T150A Antibody (Center) AP9366c
    T150A Antibody (Center) (Cat. #AP9366c) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
Western blot analysis of T150A Antibody (Center) (Cat. #AP9366c) in Jurkat cell line lysates (35ug/lane). T150A (arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q86TG1
Other Accession Q91WN2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 28835 Da
Antigen Region 81-108 aa
Additional Information
Gene ID 129303
Other Names Transmembrane protein 150A, Transmembrane protein 150, TMEM150A, TMEM150
Target/Specificity This T150A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the Central region of human T150A.
Dilution FC~~1:10~50
IHC-P~~1:50~100
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsT150A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TMEM150A
Synonyms TMEM150
Function Regulates localization of phosphatidylinositol 4-kinase (PI4K) to the plasma membrane, possibly by reducing the association of TTC7 (TTC7A or TTC7B) with the PI4K complex (PubMed:25608530). Acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis (PubMed:25608530). May also play a role in fasting-induced catabolism (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein Note=Localizes mainly at the plasma membrane; only a minor fraction localizes on intracellular structures (PubMed:25608530)
Research Areas
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$ 385.00
Cat# AP9366c
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