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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   LIPE Antibody (C-term)   

LIPE Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - LIPE Antibody (C-term) AP9376b
    Western blot analysis of LIPE Antibody (C-term) (Cat. #AP9376b) in HL-60 cell line lysates (35ug/lane). LIPE (arrow) was detected using the purified Pab.
  • IHC-P - LIPE Antibody (C-term) AP9376b
    Formalin-fixed and paraffin-embedded human testis tissue reacted with LIPE Antibody (C-term) (Cat. #AP9376b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - LIPE Antibody (C-term) AP9376b
    LIPE Antibody (C-term) (Cat. #AP9376b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q05469
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 116598 Da
Antigen Region 822-849 aa
Additional Information
Gene ID 3991
Other Names Hormone-sensitive lipase, HSL, LIPE
Target/Specificity This LIPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 822-849 amino acids from the C-terminal region of human LIPE.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLIPE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Cellular Location Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Note=Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation
Research Areas
Citations (0)

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LIPE has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.


Bezaire, V., et al. FEBS Lett. 583(18):3045-3049(2009)
Chen, H.H., et al. Am. J. Clin. Nutr. 90(2):255-262(2009)
Kuzmin, A., et al. Biol. Reprod. 81(2):319-326(2009)
Bezaire, V., et al. J. Biol. Chem. 284(27):18282-18291(2009)
Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009)

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$ 295.00
$ 99.00
Cat# AP9376b
(40 western blots)
Availability: In Stock
Bulk Size
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