|Application ||WB, IHC-P, E|
|Other Accession||P55861, P97310|
|Calculated MW||101896 Da|
|Antigen Region||649-677 aa|
|Other Names||DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, BM28, CCNL1, CDCL1, KIAA0030|
|Target/Specificity||This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from the C-terminal region of human MCM2.|
|Precautions||MCM2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BM28, CCNL1, CDCL1, KIAA0030|
|Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.|
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Provided below are standard protocols that you may find useful for product applications.
MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
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Xu, X., et al. EMBO J. 28(19):3005-3014(2009)
Liaw, K., et al. J. Cutan. Pathol. 36(10):1121-1122(2009)
Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(37):15628-15632(2009)
Chuang, L.C., et al. Mol. Cell 35(2):206-216(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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