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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   HBA2 Antibody (Center)   

HBA2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • FC - HBA2 Antibody (Center) AP9414c
    Overlay histogram showing K562 cells stained with AP9414c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9414c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - HBA2 Antibody (Center) AP9414c
    Anti-HBA2 Antibody (Center) at 1:2000 dilution + human fetal heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - HBA2 Antibody (Center) AP9414c
    Western blot analysis of HBA2 Antibody (Center) (Cat. #AP9414c) in mouse liver tissue lysates (35ug/lane). HBA2 (arrow) was detected using the purified Pab.
  • IHC-P - HBA2 Antibody (Center) AP9414c
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HBA2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P69905
Other Accession P21767
Reactivity Human, Mouse
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 15258 Da
Antigen Region 100-128 aa
Additional Information
Gene ID 3039;3040
Other Names Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain, HBA1
Target/Specificity This HBA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the Central region of human HBA2.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHBA2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HBA1
Function Involved in oxygen transport from the lung to the various peripheral tissues.
Tissue Location Red blood cells.
Research Areas
Citations (0)

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HBA2 located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.


Sessa, R., et al. Am. J. Hematol. 85(2):143-144(2010)
Sharma, V., et al. Hematology 14(5):297-300(2009)
Waye, J.S., et al. Hemoglobin 33(6):519-522(2009)
Roy, P., et al. Hemoglobin 33(6):486-491(2009)
Joly, P., et al. Hemoglobin 33(3):196-205(2009)

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$ 295.00
$ 99.00
Cat# AP9414c
(40 western blots)
Availability: In Stock
Bulk Size
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