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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IL2RG Antibody (N-term)   

IL2RG Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - IL2RG Antibody (N-term) AP9450a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized human spleen tissue labeling Pdx1 with AP9450a at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human spleen tissue. The nuclear counter stain is DAPI (blue).
  • WB - IL2RG Antibody (N-term) AP9450a
    All lanes : Anti-IL2RG Antibody (N-term) at 1:2000 dilution Lane 1: human spleen lysates Lane 2: Jurkat whole cell lysates Lane 3: Daudi whole cell lysates Lane 4: KG-1 whole cell lysates Lane 5: MOLT-4 whole cell lysates Lane 6: HL-60 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IL2RG Antibody (N-term) AP9450a
    Western blot analysis of lysate from Jurkat cell line, using IL2RG Antibody (N-term)(Cat. #AP9450a). AP9450a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - IL2RG Antibody (N-term) AP9450a
    Western blot analysis of IL2RG Antibody (N-term) (Cat. #AP9450a) in HL-60 cell line lysates (35ug/lane). IL2RG (arrow) was detected using the purified Pab.
  • FC - IL2RG Antibody (N-term) AP9450a
    IL2RG Antibody (N-term) (Cat. #AP9450a) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, E
Primary Accession P31785
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42287 Da
Antigen Region 76-101 aa
Additional Information
Gene ID 3561
Other Names Cytokine receptor common subunit gamma, Interleukin-2 receptor subunit gamma, IL-2 receptor subunit gamma, IL-2R subunit gamma, IL-2RG, gammaC, p64, CD132, IL2RG
Target/Specificity This IL2RG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-101 amino acids from the N-terminal region of human IL2RG.
Dilution IF~~1:25
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL2RG Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL2RG
Function Common subunit for the receptors for a variety of interleukins.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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Background

IL2RG is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

References

Ross, J.A., et al. J. Biol. Chem. 285(6):3582-3591(2010)
Ameratunga, R., et al. N. Z. Med. J. 122(1304):46-53(2009)
Clark, P.A., et al. Hum. Genet. 96(4):427-432(1995)

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$ 295.00
$ 99.00
Cat# AP9450a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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