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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CTLA4 Antibody (C-term)   

CTLA4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CTLA4 Antibody (C-term) AP9453b
    Western blot analysis of CTLA4 Antibody (C-term) (Cat. #AP9453b) in K562 cell line lysates (35ug/lane). CTLA4 (arrow) was detected using the purified Pab.
  • IF - CTLA4 Antibody (C-term) AP9453b
    Confocal immunofluorescent analysis of CTLA4 Antibody (C-term)(Cat#AP9453b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P16410
Other Accession P42072, Q9MYX7, P09793
Reactivity Human
Predicted Mouse, Rabbit, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24656 Da
Antigen Region 182-210 aa
Additional Information
Gene ID 1493
Other Names Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4, CD152
Target/Specificity This CTLA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-210 amino acids from the C-terminal region of human CTLA4.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTLA4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTLA4
Synonyms CD152
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Cellular Location Cell membrane; Single-pass type I membrane protein Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;
Tissue Location Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation
Research Areas
Citations (0)

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Background

CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer.

References

Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010)
Zhao, S.X., et al. PLoS ONE 5 (3), E9821 (2010)
Oaks, M.K., et al. Cell. Immunol. 201(2):144-153(2000)
Chikuma, S., et al. J. Cell. Biochem. 78(2):241-250(2000)
Magistrelli, G., et al. Eur. J. Immunol. 29(11):3596-3602(1999)

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Discontinued
Cat# AP9453b
Size:
(40 western blots)
Alternative Products:

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