|Application ||WB, IF, E|
|Other Accession||P42072, Q9MYX7, P09793|
|Predicted||Mouse, Pig, Rabbit|
|Calculated MW||24656 Da|
|Antigen Region||182-210 aa|
|Other Names||Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4, CD152|
|Target/Specificity||This CTLA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-210 amino acids from the C-terminal region of human CTLA4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CTLA4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;|
|Tissue Location||Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer.
Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010)
Zhao, S.X., et al. PLoS ONE 5 (3), E9821 (2010)
Oaks, M.K., et al. Cell. Immunol. 201(2):144-153(2000)
Chikuma, S., et al. J. Cell. Biochem. 78(2):241-250(2000)
Magistrelli, G., et al. Eur. J. Immunol. 29(11):3596-3602(1999)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.