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HSD17B3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HSD17B3 Antibody (Center) AP9515c
    All lanes : Anti-HSD17B3 Antibody (Center) at 1:1000 dilution Lane 1: human testis lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - HSD17B3 Antibody (Center) AP9515c
    Formalin-fixed and paraffin-embedded human testis tissue reacted with HSD17B3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - HSD17B3 Antibody (Center) AP9515c
    HSD17B3 Antibody (Center) (Cat. #AP9515c) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P37058
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34516 Da
Antigen Region 89-118 aa
Additional Information
Gene ID 3293
Other Names Testosterone 17-beta-dehydrogenase 3, 17-beta-hydroxysteroid dehydrogenase type 3, 17-beta-HSD 3, Testicular 17-beta-hydroxysteroid dehydrogenase, HSD17B3, EDH17B3
Target/Specificity This HSD17B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the Central region of human HSD17B3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSD17B3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSD17B3
Synonyms EDH17B3, SDR12C2
Function Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH.
Tissue Location Testis.
Research Areas
Citations (0)

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This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.


Li, J., et al. Breast Cancer Res. 12 (2), R19 (2010) :
Sata, F., et al. J Sex Med (2010) In press :
Ahn, J., et al. Hum. Mol. Genet. 18(19):3749-3757(2009)
Chakrabarti, B., et al. Autism Res 2(3):157-177(2009)
Beuten, J., et al. Cancer Epidemiol. Biomarkers Prev. 18(6):1869-1880(2009)
Andersson, S., et al. J. Clin. Endocrinol. Metab. 81(1):130-136(1996)

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$ 295.00
$ 99.00
Cat# AP9515c
(40 western blots)
Availability: In Stock
Bulk Size
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