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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ANP32A Antibody (C-term)   

ANP32A Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ANP32A Antibody (C-term) AP9520b
    All lanes : Anti-ANP32A Antibody (C-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ANP32A Antibody (C-term) AP9520b
    ANP32A Antibody (C-term) (Cat. #AP9520b) western blot analysis in K562,CEM,HL-60 cell line lysates (35ug/lane).This demonstrates the ANP32A antibody detected the ANP32A protein (arrow).
  • IHC-P - ANP32A Antibody (C-term) AP9520b
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ANP32A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P39687
Other Accession P51122
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28585 Da
Antigen Region 211-239 aa
Additional Information
Gene ID 8125
Other Names Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32, pp32, Leucine-rich acidic nuclear protein, LANP, Mapmodulin, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I, PHAPI, ANP32A, C15orf1, LANP, MAPM, PHAP1
Target/Specificity This ANP32A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the C-terminal region of human ANP32A.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANP32A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANP32A
Synonyms C15orf1, LANP, MAPM, PHAP1
Function Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase- independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1- mediated transcriptional repression.
Cellular Location Nucleus. Cytoplasm. Endoplasmic reticulum. Note=Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
Tissue Location Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
Research Areas
Citations (0)

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Background

Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. ANP32A plays a role in E4F1-mediated transcriptional repression.

References

Valdes, A.M., et al. Arthritis Rheum. 60(7):2046-2054(2009)
Pan, W., et al. J. Biol. Chem. 284(11):6946-6954(2009)
de Chiara, C., et al. FEBS J. 275(10):2548-2560(2008)
Kim, H.E., et al. Mol. Cell 30(2):239-247(2008)
Matilla, A., et al. Cerebellum 4(1):7-18(2005)

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$ 295.00
$ 99.00
Cat# AP9520b
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(40 western blots)
Availability: In Stock
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