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RPL31 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RPL31 Antibody (Center) AP9532c
    Western blot analysis of RPL31 Antibody (Center) (Cat. #AP9532c) in Hela cell line lysates (35ug/lane).RPL31 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - RPL31 Antibody (Center) AP9532c
    Formalin-fixed and paraffin-embedded human testis tissue reacted with RPL31 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - RPL31 Antibody (Center) AP9532c
    RPL31 Antibody (Center) (Cat. #AP9532c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P62899
Other Accession Q6NUH0, P62902, P62901, P62900, Q56JX3, NP_001092047, G1SHG0
Reactivity Human
Predicted Bovine, Mouse, Pig, Rabbit, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 14463 Da
Antigen Region 24-51 aa
Additional Information
Gene ID 6160
Other Names 60S ribosomal protein L31, RPL31
Target/Specificity This RPL31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the Central region of human RPL31.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL31 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL31
Function Component of the large ribosomal subunit (PubMed:23636399, PubMed:32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399, PubMed:32669547).
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL31 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm.

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)

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$ 365.00
$ 140.00
Cat# AP9532c
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