|Application ||WB, E|
|Other Accession||Q8R050, P15170|
|Calculated MW||68883 Da|
|Antigen Region||216-245 aa|
|Other Names||Eukaryotic peptide chain release factor GTP-binding subunit ERF3B, Eukaryotic peptide chain release factor subunit 3b, eRF3b, G1 to S phase transition protein 2 homolog, GSPT2, ERF3B|
|Target/Specificity||This ERF3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from the Central region of human ERF3B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ERF3B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in translation termination in response to the termination codons UAA, UAG and UGA. May play a role as a potent stimulator of the release factor activity of ETF1. Exhibits GTPase activity, which is ribosome- and ETF1-dependent. May play a role in cell cycle progression. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.|
|Tissue Location||Highly expressed in IUCC stage II colorectal cancer (CRC).|
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Provided below are standard protocols that you may find useful for product applications.
ERF3B encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability.
Cheng, Z., et al. Genes Dev. 23(9):1106-1118(2009)
Zhouravleva, G., et al. IUBMB Life 58(4):199-202(2006)
Chauvin, C., et al. Mol. Cell. Biol. 25(14):5801-5811(2005)
Hoshino, S., et al. J. Biol. Chem. 274(24):16677-16680(1999)
Hansen, L.L., et al. Cytogenet. Cell Genet. 86 (3-4), 250-251 (1999)
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