|Application ||WB, E|
|Calculated MW||43831 Da|
|Antigen Region||1-30 aa|
|Other Names||Diacylglycerol O-acyltransferase 2, Acyl-CoA retinol O-fatty-acyltransferase, ARAT, Retinol O-fatty-acyltransferase, Diglyceride acyltransferase 2, DGAT2|
|Target/Specificity||This DGAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DGAT2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DGAT2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Functions also as an acyl-CoA retinol acyltransferase (ARAT).|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein. Lipid droplet|
|Tissue Location||Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin|
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Provided below are standard protocols that you may find useful for product applications.
Acyl-CoA:diacylglycerol acyltransferase, or DGAT (EC 184.108.40.206), is responsible for the synthesis of triglycerides. It catalyzes a reaction in which diacylglycerol is covalently joined to long chain fatty acyl-CoAs.
# Kantartzis, K., et al. Clin. Sci. 116(6):531-537(2009)
# Stone, S.J., et al. J. Biol. Chem. 284(8):5352-5361(2009)
# Yen, C.L., et al. J. Lipid Res. 49(11):2283-2301(2008)
# Levin, M.C., et al. Am. J. Physiol. Endocrinol. Metab. 293 (6), E1772-E1781 (2007)
# Payne, V.A., et al. J. Biol. Chem. 282(29):21005-21014(2007)
# Yamada, S., et al. Oncogene 23(35):5901-5911(2004)
# Wakimoto, K., et al. Biochem. Biophys. Res. Commun. 310(2):296-302(2003)
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