|Application ||WB, E|
|Calculated MW||43684 Da|
|Antigen Region||130-159 aa|
|Other Names||Endonuclease 8-like 1, 322-, DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, Nei homolog 1, NEH1, Nei-like protein 1, NEIL1|
|Target/Specificity||This NEIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-159 amino acids from the Central region of human NEIL1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NEIL1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5- hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Nucleus. Chromosome. Note=During mitosis, associates with centrosomes and condensed chromatin|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).
Zhao, X., et al. Biochemistry 49(8):1658-1666(2010)
Forsbring, M., et al. Carcinogenesis 30(7):1147-1154(2009)
Couve, S., et al. J. Biol. Chem. 284(18):11963-11970(2009)
Muftuoglu, M., et al. J. Biol. Chem. 284(14):9270-9279(2009)
Horikawa, Y., et al. Clin. Cancer Res. 14(23):7956-7962(2008)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.