|Application ||WB, IHC-P, E|
|Other Accession||NP_001027024.3, NP_001036001.1|
|Calculated MW||63469 Da|
|Antigen Region||409-437 aa|
|Other Names||Protein inscuteable homolog, INSC|
|Target/Specificity||This INSC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-437 amino acids from the C-terminal region of human INSC.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||INSC Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. Involved in spindle orientation during mitosis it may regulate cell proliferation and differentiation in the developing nervous system. May play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium.|
|Cellular Location||Cytoplasm. Note=Localizes to the apical cortex of mitotic epidermal cells and to the apical side of neuronal progenitor cells.|
|Tissue Location||Isoform 1 is expressed in various tissues with stronger expression in liver, kidney and small intestine. Isoform 2 is abundantly expressed in small intestine and to a lower extent in lung and pancreas.|
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Provided below are standard protocols that you may find useful for product applications.
In Drosophila, neuroblasts divide asymmetrically into another neuroblast at the apical side and a smaller ganglion mother cell on the basal side. Cell polarization is precisely regulated by 2 apically localized multiprotein signaling complexes that are tethered by Inscuteable, which regulates their apical localization (Izaki et al., 2006 [PubMed 16458856]).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Vural, A., et al. Mol. Cell. Biol. 30(6):1528-1540(2010)
Rivadeneira, F., et al. Nat. Genet. 41(11):1199-1206(2009)
Izaki, T., et al. Biochem. Biophys. Res. Commun. 341(4):1001-1006(2006)
Katoh, M., et al. Int. J. Mol. Med. 11(1):111-116(2003)
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