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ZN473 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ZN473 Antibody (N-term) AP9752a
    Western blot analysis of ZN473 Antibody (N-term) (Cat. #AP9752a) in NCI-H460 cell line lysates (35ug/lane). ZN473 (arrow) was detected using the purified Pab.
  • FC - ZN473 Antibody (N-term) AP9752a
    ZN473 Antibody (N-term) (Cat. #AP9752a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q8WTR7
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 100182 Da
Antigen Region 178-207 aa
Additional Information
Gene ID 25888
Other Names Zinc finger protein 473, Zinc finger protein 100 homolog, Zfp-100, ZNF473, KIAA1141, ZFP100
Target/Specificity This ZN473 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human ZN473.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZN473 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZNF473
Synonyms KIAA1141, ZFP100
Function Involved in histone 3'-end pre-mRNA processing by associating with U7 snRNP and interacting with SLBP/pre-mRNA complex. Increases histone 3'-end pre-mRNA processing but has no effect on U7 snRNP levels, when overexpressed. Required for cell cycle progression from G1 to S phases.
Cellular Location Nucleus. Note=Stable component of Cajal bodies (CBs). Colocalizes with SMN, coilin and U7 snRNA
Citations ( 0 )

References

Wagner, E.J., et al. Mol. Cell. Biol. 26(17):6702-6712(2006)
Wagner, E.J., et al. RNA 12(7):1206-1218(2006)
Pillai, R.S., et al. Genes Dev. 17(18):2321-2333(2003)
Dominski, Z., et al. Genes Dev. 16(1):58-71(2002)

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$ 295.00
$ 99.00
Cat# AP9752a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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