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GLG1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - GLG1 Antibody (C-term) AP9839b
    All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GLG1 Antibody (C-term) AP9839b
    All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution Lane 1: MCF-7 lysate Lane 2: HepG2 whole cell lysate Lane 3: T47D whole cell lysate Lane 4: mouse cerebellum lysate Lane 5: human placenta lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GLG1 Antibody (C-term) AP9839b
    All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: mouse cerebellum lysate Lane 3: MCF-7 whole cell lysate Lane 4: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GLG1 Antibody (C-term) AP9839b
    GLG1 Antibody (C-term) (Cat. #AP9839b) western blot analysis in HepG2,T47D cell line lysates (35ug/lane).This demonstrates the GLG1 antibody detected the GLG1 protein (arrow).
  • WB - GLG1 Antibody (C-term) AP9839b
    Western blot analysis of GLG1 Antibody (C-term) (Cat. #AP9839b) in mouse cerebellum tissue lysates (35ug/lane). GLG1 (arrow) was detected using the purified Pab.
  • IHC-P - GLG1 Antibody (C-term) AP9839b
    GLG1 Antibody (C-term) (Cat. #AP9839b) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q92896
Other Accession Q62638, Q61543, Q9Z1E9, Q02391
Reactivity Human, Mouse
Predicted Rat, Hamster, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 134552 Da
Antigen Region 1152-1179 aa
Additional Information
Gene ID 2734
Other Names Golgi apparatus protein 1, CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand 1, ESL-1, Golgi sialoglycoprotein MG-160, GLG1, CFR1, ESL1, MG160
Target/Specificity This GLG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1179 amino acids from the C-terminal region of human GLG1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLG1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GLG1
Synonyms CFR1, ESL1, MG160
Function Binds fibroblast growth factor and E-selectin (cell- adhesion lectin on endothelial cells mediating the binding of neutrophils).
Cellular Location Golgi apparatus membrane; Single-pass type I membrane protein
Tissue Location Widely expressed. Highest levels in pancreas, skeletal muscle, placenta, heart, testis and ovary. Also found in the kidney, liver, lung and brain.
Research Areas
Citations (0)

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References

Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)
Kibriya, M.G., et al. Breast Cancer Res. Treat. 114(3):463-477(2009)
Antoine, M., et al. Oncol. Rep. 21(2):357-362(2009)
Ahn, J., et al. J. Cell. Sci. 118 (PT 8), 1725-1731 (2005)
Bouwmeester, T., et al. Nat. Cell Biol. 6(2):97-105(2004)

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$ 295.00
$ 99.00
Cat# AP9839b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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