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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   HADHB Antibody (C-term)   

HADHB Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HADHB Antibody (C-term) AP9859b
    Western blot analysis of HADHB Antibody (C-term) (Cat. #AP9859b) in Jurkat cell line lysates (35ug/lane). HADHB (arrow) was detected using the purified Pab.
  • FC - HADHB Antibody (C-term) AP9859b
    HADHB Antibody (C-term) (Cat. #AP9859b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P55084
Other Accession Q8HXX4, O46629
Reactivity Human
Predicted Bovine, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51294 Da
Antigen Region 315-342 aa
Additional Information
Gene ID 3032
Other Names Trifunctional enzyme subunit beta, mitochondrial, TP-beta, 3-ketoacyl-CoA thiolase, Acetyl-CoA acyltransferase, Beta-ketothiolase, HADHB
Target/Specificity This HADHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-342 amino acids from the C-terminal region of human HADHB.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHADHB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HADHB
Cellular Location Mitochondrion. Mitochondrion inner membrane Mitochondrion outer membrane Endoplasmic reticulum
Research Areas
Citations (0)

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Background

HADHB encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. Mutations in this gene result in trifunctional protein deficiency. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.

References

Purevsuren, J., et al. Mol. Genet. Metab. 98(4):372-377(2009)
Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008)
Wang, R., et al. Zhonghua Fu Chan Ke Za Zhi 41(10):672-675(2006)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)

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Cat# AP9859b
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(40 western blots)
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