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GSTK1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GSTK1 Antibody (Center) AP9867c
    Western blot analysis of GSTK1 Antibody (Center) (Cat. #AP9867c) in mouse liver tissue lysates (35ug/lane). GSTK1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - GSTK1 Antibody (Center) AP9867c
    GSTK1 Antibody (Center) (Cat. #AP9867c) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSTK1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - GSTK1 Antibody (Center) AP9867c
    GSTK1 Antibody (Center) (Cat. #AP9867c) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9Y2Q3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25497 Da
Antigen Region 93-120 aa
Additional Information
Gene ID 373156
Other Names Glutathione S-transferase kappa 1, GST 13-13, GST class-kappa, GSTK1-1, hGSTK1, Glutathione S-transferase subunit 13, GSTK1
Target/Specificity This GSTK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the Central region of human GSTK1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSTK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSTK1
Function Glutathione S-transferase that catalyzes the conjugation of glutathione to exogenous and endogenous compounds (PubMed:14709161, PubMed:14742434). Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB) (PubMed:14709161).
Cellular Location Peroxisome.
Tissue Location Ubiquitous..
Research Areas
Citations (0)
citation

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Background

GSTK1 encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.

References

Santos, G.S., et al. Int. J. Mol. Med. 24(3):393-399(2009)
Aydos, S.E., et al. Fertil. Steril. 92(2):541-547(2009)
Gao, F., et al. Endocr. J. 56(3):487-494(2009)
Katoh, T., et al. Pharmacogenomics 9(1):93-104(2008)
Li, J., et al. Protein Sci. 14(9):2361-2369(2005)

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$ 365.00
$ 140.00
Cat# AP9867c
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