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AIM2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AIM2 Antibody (N-term) AP9876a
    Western blot analysis of AIM2 Antibody (N-term) (Cat. #AP9876a) in MCF-7, HL-60, K562, Hela cell line lysates (35ug/lane). AIM2 (arrow) was detected using the purified Pab.
  • FC - AIM2 Antibody (N-term) AP9876a
    AIM2 Antibody (N-term) (Cat. #AP9876a) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - AIM2 Antibody (N-term) AP9876a
    Confocal immunofluorescent analysis of AIM2 Antibody (N-term)(Cat#AP9876a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IF, E
Primary Accession O14862
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38954 Da
Antigen Region 4-32 aa
Additional Information
Gene ID 9447
Other Names Interferon-inducible protein AIM2, Absent in melanoma 2, AIM2
Target/Specificity This AIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human AIM2.
Dilution WB~~1:1000
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAIM2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AIM2
Function Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.
Cellular Location Nucleus. Cytoplasm. Note=Activated inflammasomes can aggregate in the cytosol as speck-like particles
Tissue Location Expressed in spleen, small intestine, peripheral blood leukocytes, and testis
Research Areas
Citations (0)

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Background

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.

References

Patsos, G., et al. Int. J. Cancer 126(8):1838-1849(2010)
Patsos, G., et al. Glycobiology 19(7):726-734(2009)
Fernandes-Alnemri, T., et al. Nature 458(7237):509-513(2009)
Hornung, V., et al. Nature 458(7237):514-518(2009)
Woerner, S.M., et al. Genes Chromosomes Cancer 46(12):1080-1089(2007)
Cresswell, K.S., et al. Biochem. Biophys. Res. Commun. 326(2):417-424(2005)

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Cat# AP9876a
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(40 western blots)
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