|Application ||WB, E|
|Other Accession||Q4R8H2, Q1RMH9|
|Calculated MW||71996 Da|
|Antigen Region||226-255 aa|
|Other Names||DnaJ homolog subfamily C member 2, M-phase phosphoprotein 11, Zuotin-related factor 1, DnaJ homolog subfamily C member 2, N-terminally processed, DNAJC2, MPHOSPH11, MPP11, ZRF1|
|Target/Specificity||This DNAJC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-255 amino acids from the Central region of human DNAJC2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DNAJC2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||MPHOSPH11, MPP11, ZRF1|
|Function||Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb- repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.|
|Cellular Location||Nucleus. Cytoplasm, cytosol|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6.
Hatzold, J., et al. PLoS Biol. 6 (4), E84 (2008) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Otto, H., et al. Proc. Natl. Acad. Sci. U.S.A. 102(29):10064-10069(2005)
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