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5NT1A Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP9917a 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP9917a-ev20 Ev20 size 100 ul In Stock $ 95.00 Add to cart
  • Specification
  • Citiations : 0
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  • Protocols
  • Backgrounds

5NT1A Antibody (N-term) - Product info

ApplicationWB, IHC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionQ9BXI3
ReactivityHuman, Mouse
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW41021 Da

5NT1A Antibody (N-term) - Additional info

Gene ID 84618
Other Names
NT5C1A; Cytosolic 5'-nucleotidase 1A; Cytosolic 5'-nucleotidase IA
Target/Specificity
This 5NT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98~127 amino acids from the N-terminal region of human 5NT1A.
Dilution
WB~~1:100~500
IHC~~1:50~100
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
5NT1A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

5NT1A Antibody (N-term) - Protein Information

Name NT5C1A
Function
Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia
Cellular Location
Cytoplasm.
Tissue Location
Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas

5NT1A Antibody (N-term) - Related products

AP9917a: 5NT1A Antibody (N-term)

DC09287: Human NT5C1A cDNA Clone

BP9917a: 5NT1A Antibody (N-term) Blocking Peptide

5NT1A Antibody (N-term) - Application data

  • Western blot analysis of 5NT1A Antibody (N-term) (Cat. #AP9917a) in mouse heart tissue lysates (35ug/lane). 5NT1A (arrow) was detected using the purified Pab.

  • 5NT1A Antibody (N-term) (Cat. #AP9917a) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the 5NT1A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

5NT1A Antibody (N-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates.

REFERENCES

Landers, J.E., et al. Proc. Natl. Acad. Sci. U.S.A. 106(22):9004-9009(2009) Lechward, K., et al. Acta Biochim. Biophys. Sin. (Shanghai) 41(4):280-284(2009) Fyrberg, A., et al. Nucleosides Nucleotides Nucleic Acids 25 (9-11), 1201-1204 (2006) :