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DEN1A Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DEN1A Antibody (C-term) AP9919b
    Anti-DEN1A Antibody (C-term) at 1:500 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 111 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - DEN1A Antibody (C-term) AP9919b
    DEN1A Antibody (C-term) (Cat. #AP9919b) IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEN1A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - DEN1A Antibody (C-term) AP9919b
    DEN1A Antibody (C-term) (Cat. #AP9919b) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8TEH3
Other Accession Q8K382
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 110577 Da
Antigen Region 576-605 aa
Additional Information
Gene ID 57706
Other Names DENN domain-containing protein 1A, Connecdenn 1, Connecdenn, Protein FAM31A, DENND1A, FAM31A, KIAA1608
Target/Specificity This DEN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-605 amino acids from the C-terminal region of human DEN1A.
Dilution WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDEN1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms FAM31A, KIAA1608
Function Guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles.
Cellular Location Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein. Cell junction, synapse, presynaptic cell membrane
Research Areas
Citations (0)

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Allaire, P.D., et al. J. Neurosci. 26(51):13202-13212(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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$ 295.00
$ 99.00
Cat# AP9919b
(40 western blots)
Availability: In Stock
Bulk Size
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