|Application ||WB, IHC-P, FC, IF, E|
|Calculated MW||43626 Da|
|Antigen Region||184-212 aa|
|Other Names||T-cell surface glycoprotein CD1e, membrane-associated, hCD1e, R2G1, CD1e, T-cell surface glycoprotein CD1e, soluble, sCD1e, CD1E|
|Target/Specificity||This CD1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-212 amino acids from the Central region of human CD1E.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD1E Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||T-cell surface glycoprotein CD1e, soluble binds diacetylated lipids, including phosphatidyl inositides and diacylated sulfoglycolipids, and is required for the presentation of glycolipid antigens on the cell surface. The membrane- associated form is not active.|
|Cellular Location||T-cell surface glycoprotein CD1e, membrane- associated: Golgi apparatus membrane; Single-pass type I membrane protein. Early endosome. Late endosome. Note=Predominantly localized in the trans-Golgi network in immature dendritic cells, and as a cleaved, soluble protein in the lysosome lumen of mature dendritic cells|
|Tissue Location||Expressed on cortical thymocytes, dendritic cells, Langerhans cells, on certain T-cell leukemias, and in various other tissues.|
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Provided below are standard protocols that you may find useful for product applications.
CD1E encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members.
Maitre, B., et al. Biochem. J. 419(3):661-668(2009)
Kuijf, M.L., et al. J. Neuroimmunol. 205 (1-2), 110-112 (2008)
Maitre, B., et al. Traffic 9(4):431-445(2008)
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