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DNAJC6 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DNAJC6 Antibody (Center) AP9982A
    DNAJC6 Antibody (Center) (Cat. #AP9982a) western blot analysis in K562(lane 1),HL-60 cell line(lane 2) and mouse brain tissue(lane 3) lysates (35ug/lane).This demonstrates the DNAJC6 antibody detected the DNAJC6 protein (arrow).
  • IHC-P - DNAJC6 Antibody (Center) AP9982A
    DNAJC6 Antibody (Center) (Cat. #AP9982a) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNAJC6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - DNAJC6 Antibody (Center) AP9982A
    DNAJC6 Antibody (Center) (Cat. #AP9982a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75061
Other Accession Q80TZ3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 99997 Da
Antigen Region 254-281 aa
Additional Information
Gene ID 9829
Other Names Putative tyrosine-protein phosphatase auxilin, DnaJ homolog subfamily C member 6, DNAJC6, KIAA0473
Target/Specificity This DNAJC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-281 amino acids from the Central region of human DNAJC6.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNAJC6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA0473
Function Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons (By similarity).
Tissue Location Expressed in various brain regions, including cerebellum, corpus callosum, cortex, striatum, brainstem, pons, putamen, spinal cord and substantia nigra. Very low expression in non-neural tissues such as leukocytes, liver, adipose tissue, skeletal muscle and bone marrow.
Citations (0)

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DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain


Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Hirst, J., et al. Traffic 9(8):1354-1371(2008)

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Cat# AP9982A
(40 western blots)
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