|Application ||WB, ICC, E, IP|
|Other Accession||NP_009330, 6274552|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 91 kDa |
Observed: 90 kDa
|Application Notes||STAT1 alpha antibody can be used for Western blot at 0.5 - 1 µg and for immunoprecipitation at 2 to 4 µg per sample. Antibody can also be used for immunocytochemistry starting at 10 µg/mL.|
|Other Names||STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91, signal transducer and activator of transcription 1, 91kDa|
|Target/Specificity||STAT1; At least two isoforms of STAT1 are known to exist; this antibody will only recognize the larger isoform.|
|Reconstitution & Storage||STAT1 alpha antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||STAT1 alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN- stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN- gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4|
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Provided below are standard protocols that you may find useful for product applications.
STAT1 alpha Antibody: STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-α, IFN-β, IFN-γ and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1).
Leonard WJ and O'Shea JJ. Jaks and STATs: biological implications. Annu. Rev. Immunol. 1998; 16:293-322.
Schindler C and Darnell JE Jr. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu. Rev. Biochem. 1995; 64:621-51.
Darnell JE Jr. STATs and gene regulation. Science 1997; 277:1630-5.
Schindler C, Fu XY, Improta T, et al. Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon α. Proc. Natl. Acad. Sci. USA 1992; 89:7836-9.
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