|Application ||WB, ICC, E|
|Other Accession||NP_542970, 18426911|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||75 - 110 kDa|
|Application Notes||SIRP alpha antibody can be used for Western blot at 0.5 - 1 µg/mL. 75 - 110 kDa bands should be detected. Antibody can also be used for immunocytochemistry starting at 1 µg/mL.|
|Other Names||SIRP alpha Antibody: BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1, BIT, MYD1, Tyrosine-protein phosphatase non-receptor type substrate 1, Brain Ig-like molecule with tyrosine-based activation motifs, SHP substrate 1, signal-regulatory protein alpha|
|Target/Specificity||SIRPA; Recognizes SIRP alpha 1, 2 and 3.|
|Reconstitution & Storage||SIRP alpha antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SIRP alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP|
|Function||Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
SIRP alpha Antibody: Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.
Kharitonenkov A, et al. Nature 1997;386:181-186.
Fujioka Y, et al. Mol Cell Biol 1996;16:6887-6899
Yamao T, et al. Biochem Biophys Res Commun 1997;231:61-67
Brooke GP, et al. Eur J Immunol 1998;28:1-11
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