|Application ||WB, ICC, E|
|Other Accession||AAC52036, 2145080|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 51 kDa |
Observed: 52 kDa
|Application Notes||GFR alpha 2 antibody can be used for detection of GFR alpha 2 by Western blot at 1 µg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 µg/mL.|
|Other Names||GFR alpha 2 Antibody: NTNRA, RETL2, TRNR2, GDNFRB, NRTNR-ALPHA, GDNF family receptor alpha-2, GDNF receptor beta, GDNF receptor alpha-2, GDNF family receptor alpha 2|
|Reconstitution & Storage||GFR alpha 2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||GFR alpha 2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||GDNFRB, RETL2, TRNR2|
|Function||Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor|
|Tissue Location||Isoform 1 is found in both brain and placenta.|
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Provided below are standard protocols that you may find useful for product applications.
GFR alpha 2 Antibody: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNRalpha, which is a cell surface receptor for NTN. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently. NTNRalpha was also termed GDNFRβ, Ret ligand 2 (RETL2) or TGFbeta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. GFRα-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRα-2, and the Ret PTK form a complex to transduce NTN signal and to mediate NTN function.
Kotzbauer PT, Lampe PA, Heuckeroth RO, et al. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996; 384:467-70.
Klein RD, Sherman D, Ho WH, et al. A GPI-linked protein that interacts with Ret to form a candidate neurturin receptor. Nature 1997; 387:717-21.
Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997; 387:721-4.
Baloh RH, Tansey MG, Golden JP, et al. TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret. Neuron 1997; 18:793-802.
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