|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_034410, 14587|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 44 kDa |
Observed: 43 kDa
|Application Notes||GFR alpha 3 antibody can be used for detection of GFR alpha 3 expression by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.|
|Other Names||GFR alpha 3 Antibody: Y15110, GFRalpha3, GDNF family receptor alpha-3, GDNF receptor alpha-3, glial cell line derived neurotrophic factor family receptor alpha 3|
|Target/Specificity||GFR alpha 3 antibody was raised against a 14 amino acid peptide near the center of murine GFR alpha 3.|
The immunogen is located within the last 50 amino acids of GFR alpha 3.
|Reconstitution & Storage||GFR alpha 3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||GFR alpha 3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for the glial cell line-derived neurotrophic factor, artemin. Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase (By similarity).|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor|
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Provided below are standard protocols that you may find useful for product applications.
GFR alpha 3 Antibody: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRα and Ret receptor tyrosine kinase GFRbeta. Following the findings of GFRα-1 and -2, a novel receptor in GFRα family was identified from human and mouse and designated GFRα-3. GFRα-3 binds persephin, thus, persephin, GFRα-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
Milbrandt J, de Sauvage FJ, Fahrner TJ, et al. Persephin, a novel neurotrophic factor related to GDNF and neurturin. Neuron 1998; 20:245-253.
Nomeclature Committee. Nomeclature of GPI-linked receptors for the GDNF ligand family. GFRa. Neuron 1997; 19:485.
Jing S, Yu Y, Fang M, et al. GFRα-2 and GFRα-3 are two new receptors for ligands of the DGNF family. J. Biol. Chem. 1997; 272:33111-7.
Baloh RH, Gorodinsky A, Golden JP, et al. GFRα3 is an orphan member of the GDNF/neurturin/persephin receptor family. Proc. Natl. Acad. Sci. USA 1998; 95:5801-6.
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