|Application ||WB, IHC-P, IF, E|
|Other Accession||AF179286, 23429|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||32 kDa|
|Application Notes||DEDAF can be used for detection of DEDAF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Other Names||DEDAF Antibody: AAP1, DEDAF, YEAF1, RING1 and YY1-binding protein, Apoptin-associating protein 1, APAP-1, RING1 and YY1 binding protein|
|Target/Specificity||DEDAF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DEDAF.|
The immunogen is located within the last 50 amino acids of DEDAF.
|Reconstitution & Storage||DEDAF antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||DEDAF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.|
|Cellular Location||Nucleus. Cytoplasm. Nucleus, nucleoplasm. Note=Primarily found in the nucleus. Detected in a punctate pattern likely to represent Polycomb group (PcG) bodies (By similarity).|
|Tissue Location||Widely expressed with highest levels in lymphoid tissues and placenta.|
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Provided below are standard protocols that you may find useful for product applications.
DEDAF Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
Zheng L, Schickling O, Peter ME, et al. The death effector domain-associated factor (DEDAF) plays distinct regulatory roles in the nucleus and cytoplasm. J. Biol. Chem. 2001; 276:31945-52.
Garcia E, Marcos-Gutierrez C, del Mar Lorente M, et al. RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. EMBO J. 1999;18:3404-18.
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