Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Nephrin Antibody   

Nephrin Antibody

  • WB - Nephrin Antibody ASC10103
    Western blot analysis of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 µg/mL in the (A) absence and (B) presence of blocking peptide.
  • IHC - Nephrin Antibody ASC10103
    Immunohistochemistry of Nephrin in mouse kidney tissue with Nephrin antibody at 1 µg/mL.
  • IF - Nephrin Antibody ASC10103
    Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 10 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60500
Other Accession NP_004637, 4758822
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 134742 Da
Application Notes Nephrin antibody can be used for detection of Nephrin by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 1 µg/mL. For immunofluorescence start at 10 µg/mL.
Additional Information
Gene ID 4868
Other Names Nephrin Antibody: CNF, NPHN, nephrin, Nephrin, Renal glomerulus-specific cell adhesion receptor, nephrosis 1, congenital, Finnish type (nephrin)
Target/Specificity NPHS1;
Reconstitution & Storage Nephrin antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsNephrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NPHS1
Synonyms NPHN
Function Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane
Tissue Location Specifically expressed in podocytes of kidney glomeruli
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.


Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein - Nephrin - is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582.
Tryggvason K. Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm. J. Am. Soc. Nephrol. 1999; 10:2440-5.
Tryggvason K and Wartiovaara J. Molecular basis of glomerular permselectivity. Curr, Opin. Nephrol. Hypertens. 2001; 10:543-9.


Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

$ 310.00
Cat# ASC10103
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions