|Application ||WB, IHC-P, E|
|Other Accession||O95381, 50400606|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 27, 36, 67 kDa |
Observed: 71 kDa
|Application Notes||AIF antibody can be used for detection of AIF by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL.|
|Other Names||AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1, connector enhancer of kinase suppressor of Ras 1|
|Target/Specificity||CNKSR1; At least five isoforms of AIF are known to exist; this antibody will detect all isoforms except isoform 5.|
|Reconstitution & Storage||AIF antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||AIF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.
Zamzami N and Kroemer G. Condensed matter in cell death. Nature 1999; 401:127-8.
Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999; 397:441-6.
Daugas E, Susin SA, Zamzami N, et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 2000; 14:729-39.
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