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FLASH Antibody

  • WB - FLASH Antibody ASC10105
    Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 µg/mL.
  • ICC - FLASH Antibody ASC10105
    Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 µg/mL.
  • IF - FLASH Antibody ASC10105
    Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UKL3
Other Accession AF165161, 5533376
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 220 kDa
Application Notes FLASH antibody can be used for detection of FLASH by Western blot at 0.5 µg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 10 µg/mL.
Additional Information
Gene ID 9994
Other Names FLASH Antibody: CED-4, FLASH, RIP25, KIAA1315, CASP8-associated protein 2, FLICE-associated huge protein, caspase 8 associated protein 2
Target/Specificity CASP8AP2;
Reconstitution & Storage FLASH antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsFLASH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CASP8AP2 (HGNC:1510)
Function Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF- kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.
Cellular Location Cytoplasm. Nucleus. Nucleus, PML body. Mitochondrion. Note=Exported from the nucleus to the mitochondria upon FAS activation
Research Areas
Citations (0)

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FLASH Antibody: A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.


Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K, Yonehara S. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature 1999;398:777-85
Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3
Medema JP. Apoptosis. Life and death in a FLASH. Nature 1999;398:756-7 (WD0800)

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$ 310.00
Cat# ASC10105
(40 western blots)
Availability: 5-7days
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