|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_009130, 5225377|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 66 kDa |
Observed: 70 kDa
|Application Notes||IRAK-M antibody can be used for detection of IRAK-M by Western blot at 1 µg/mL. IRAK-M antibody can also detect IRAK-M by immunohistochemistry at 2 µg/mL. For immunofluorescence start at 10 µg/mL.|
|Other Names||IRAK-M Antibody: ASRT5, IRAKM, Interleukin-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, IRAK-3, interleukin-1 receptor-associated kinase 3|
|Target/Specificity||IRAK3; IRAK-M antibody is predicted to have no cross reactivity to other members of the IRAK family.|
|Reconstitution & Storage||IRAK-M antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||IRAK-M Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits dissociation of IRAK1 and IRAK4 from the Toll- like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.|
|Tissue Location||Expressed predominantly in peripheral blood lymphocytes.|
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Provided below are standard protocols that you may find useful for product applications.
IRAK-M Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-betaB by IL-1/Toll receptors, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-betaB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.
Cao Z, Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.
Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.
Wesche H, Gao X, Li X, et al. IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family. J. Biol. Chem. 1999; 274:19403-10.
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