|Application ||WB, IHC-P, E, IP|
|Other Accession||NP_075721, 8953908|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 26 kDa |
Observed: 25 kDa
|Application Notes||Smac antibody can be used for detection of Smac/DIABLO by Western blot at 1 µg/mL. Smac antibody can also be used to detect Smac/DIABLO by immunohistochemistry at 5 µg/mL.|
|Other Names||Smac Antibody: Smac, AU040403, 0610041G12Rik, 1700006L01Rik, Smac, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, diablo homolog (Drosophila)|
|Reconstitution & Storage||Smac antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Smac Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases (By similarity).|
|Cellular Location||Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis|
|Tissue Location||Highest expression found in heart, liver, kidney and testis.|
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Provided below are standard protocols that you may find useful for product applications.
Smac Antibody: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
Du C, Fang M, Li Y, et al. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell 2000; 102:33-42.
Verhagen AM, Ekert PG, Pakusch M, et al. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell 2000;102:43-53.
Srinivasula SM, Datta P, Fan XJ, et al. Molecular Determinants of the Caspase-promoting Activity of Smac/DIABLO and Its Role in the Death Receptor Pathway. J. Biol. Chem. 2000; 275:36152-7.
Chai J, Du C, Wu JW, et al. Structural and biochemical basis of apoptotic activation by Smac/DIABLO. Nature 2000; 406:855-62.
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