|Application ||WB, ICC, E|
|Other Accession||NP_068575, 11141875|
|Calculated MW||Predicted: 18 kDa |
Observed: 18 kDa
|Application Notes||IL-21 antibody can be used for detection of IL-21 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 2 µg/mL.|
|Other Names||IL-21 Antibody: Za11, IL-21, Interleukin-21, Za11, interleukin 21|
|Target/Specificity||IL21; At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.|
|Reconstitution & Storage||IL-21 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||IL-21 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.|
|Tissue Location||Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes|
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Provided below are standard protocols that you may find useful for product applications.
IL-21 Antibody: A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature 2000; 408:57-63.
Ozaki K, Kikly K, Michalovich D, et al. Cloning of a type I cytokine receptor most related to the IL-2 receptor β chain. Proc. Natl. Acad. Sci. USA. 2000; 97:11439-44.
Asao H, Okuyama C, Kumaki S, et al. The common γ-chain is an indispensable subunit of the IL-21 receptor complex. J. Immunol. 2001; 167:1-5.
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