|Application ||WB, IF, ICC, E|
|Other Accession||AAG50014, 12247742|
|Calculated MW||48933 Da|
|Application Notes||CARD8 antibody can be used for detection of CARD8 by Western blot at 2 and 4 µg/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Other Names||CARD8 Antibody: NDPP, DACAR, DAKAR, NDPP1, TUCAN, CARDINAL, KIAA0955, Caspase recruitment domain-containing protein 8, Apoptotic protein NDPP1, caspase recruitment domain family, member 8|
|Reconstitution & Storage||CARD8 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CARD8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow|
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Provided below are standard protocols that you may find useful for product applications.
CARD8 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-κB activation.
Scholl FA, McLoghlin P, Ehler E, et al. DRAL is a p53-responsive gene whose four and a half LIM domain protein product induces apoptosis. J. Cell Biol. 2000; 151:495-506.
Stil R, Leonardi A, Formisano L, et al. TUCAN/CARDINAL and DRAL participate in a B activation. FEBS 2002;kcommon pathway for modulation of NF- 521:165-9.
Razmara M, Srinivasula SM, Wang L, et al. CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis. J. Biol. Chem. 2002; 277:13952-8.
Pathan N, Marusawa H, Krajewska M, et al. TUCAN, an antiapoptotic caspase-assoicated recruitment domain family protein overexpressed in cancer. J. Biol. Chem. 2001; 276:32220-9.
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