|Application ||WB, IF, ICC, E|
|Other Accession||NP_001217, 14916483|
|Calculated MW||33310 Da|
|Application Notes||Casp-6 antibody can be used for the detection of Caspase-6 by Western blot at 1 and 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 1 µg/mL. For immunofluorescence start at 10 µg/mL.|
|Other Names||Caspase-6 Antibody: MCH2, MCH2, Caspase-6, Apoptotic protease Mch-2, CASP-6, caspase 6, apoptosis-related cysteine peptidase|
|Target/Specificity||CASP6; Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.|
|Reconstitution & Storage||Caspase-6 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Caspase-6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.|
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Provided below are standard protocols that you may find useful for product applications.
Caspase-6 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-6 is an executioner caspase that was identified based on its homology with human caspases 2 and 3 as well as the C. elegans cell death protein CED-3. It possesses two isoforms, of which only the longer form possesses protease activity. Caspase-6 is highly expressed in adult brain and may play a role in several neuronal pathologies.
Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.
Fernandes-Alnemri T, Litwack G, and Alnemri ES. Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family. Cancer Res. 1995; 55:2737-42.
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