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Bif Antibody

  • WB - Bif Antibody ASC10425
    Western blot analysis of BIF in HeLa cell lysate with BIF antibody at (A) 1 and (B) 2 µg/mL.
  • ICC - Bif Antibody ASC10425
    Immunocytochemistry of BIF in HeLa with BIF antibody at 10 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y371
Other Accession AAK27365, 13469879
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 40796 Da
Application Notes BIF antibody can be used for the detection of BIF by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL.
Additional Information
Gene ID 51100
Other Names Bif Antibody: Bif-1, CGI-61, PPP1R70, dJ612B15.2, KIAA0491, Endophilin-B1, Bax-interacting factor 1, Bif-1, SH3-domain GRB2-like endophilin B1
Target/Specificity SH3GLB1;
Reconstitution & Storage Bif antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsBif Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SH3GLB1
Synonyms KIAA0491
Function May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion.
Cellular Location Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Note=Association with the Golgi apparatus depends on the cell type.
Tissue Location Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
Research Areas
Citations (0)

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Bif Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.


Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
Oltvai ZN, Milliman CL, and Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993; 74:609-19.
Cuddeback SM, Yamaguchi H, Komatsu K, et al. Molecular cloning and characterization of bif-1. J. Biol. Chem.2001; 276:20559-65.
Takahashi Y, Karbowski M, Yamaguchi H, et al. Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis. Mol. Cell. Biol. 2005; 25:9369-82.

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$ 310.00
Cat# ASC10425
(40 western blots)
Availability: 5-7days
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