|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_004040, 4757840|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 18, 19 kDa |
Observed: 22 kDa
|Application Notes||Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 - 2 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Other Names||Bfl-1 Antibody: GRS, BFL1, ACC-1, ACC-2, HBPA1, BCL2L5, GRS, Bcl-2-related protein A1, Bcl-2-like protein 5, Bcl2-L-5, BCL2-related protein A1|
|Target/Specificity||BCL2A1; At least two isoforms of Bfl-1 are known to exist; this antibody will detect both isoforms.|
|Reconstitution & Storage||Bfl-1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Bfl-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BCL2L5, BFL1, GRS, HBPA1|
|Function||Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity).|
|Tissue Location||Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies|
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Provided below are standard protocols that you may find useful for product applications.
Bfl-1 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist.
Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
Choi SS, Park IC, Yun JW, et al. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene 1995; 11:1693-8.
Kim JK, Kim KD, Lee E, et al. Up-regulation of Bfl-1/A1 via NF-κB activation in cisplatin-resistant human bladder cancer cell line. Cancer Lett. 2004; 212:61-70.
Zhang H, Cowan-Jacob SW, Simonen M, et al. Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells. J. Biol. Chem.2000; 275:11092-9.
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