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APH1 Antibody

  • WB - APH1 Antibody ASC10486
    Western blot analysis of APH1 in human brain tissue lysate with APH1 antibody at (A) 0.5 and (B) 1 µg/mL.
  • IHC - APH1 Antibody ASC10486
    Immunohistochemistry of APH1 in human brain tissue with APH1 antibody at 5 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96BI3
Other Accession AAH08732, 14250557
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 28996 Da
Application Notes APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 1 µg/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.
Additional Information
Gene ID 51107
Other Names APH1 Antibody: APH-1, APH-1A, CGI-78, 6530402N02Rik, PSF, UNQ579/PRO1141, Gamma-secretase subunit APH-1A, Aph-1alpha, APH-1a, anterior pharynx defective 1 homolog A (C. elegans)
Target/Specificity APH1A;
Reconstitution & Storage APH1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsAPH1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APH1A
Synonyms PSF
Function Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi- pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi
Tissue Location Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
Research Areas
Citations (0)

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APH1 Antibody: APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer's disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed.


Goutte C, Tsunozaki M, Hale VA, et al. APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos. Proc. Natl. Acad. Sci. USA2002; 99:775-9.
Periz G and Fortini ME. Functional reconstitution of γsecretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.
Selkoe DJ. The cell biology of βamyloid precursor protein and presenilin in Alzheimer’s disease. Trends Cell Biol. 1998; 8:447-53.
Ma G, Li T, Price DL, et al. APH-1a is the principal mammalian aph-1 isoform present in g-secretase complexes during embryonic development. Neuro. Dis. 2005; 25:192-8.

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$ 310.00
Cat# ASC10486
(40 western blots)
Availability: 5-7days
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