|Application ||WB, IF, E|
|Other Accession||AAH05942, 13543577|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||10059 Da|
|Application Notes||BANF1 antibody can be used for detection of BANF1 by Western blot at 0.5 - 2 µg/mL. Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 20 µg/mL.|
|Other Names||BANF1 Antibody: BAF, NGPS, BCRP1, D14S1460, BAF, BCRG1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1, barrier to autointegration factor 1|
|Reconstitution & Storage||BANF1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||BANF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging.|
|Cellular Location||Barrier-to-autointegration factor: Nucleus Cytoplasm. Chromosome. Nucleus envelope. Note=Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase The phosphorylated form (by VRK1 or vaccinia virus B1 kinase) shows a cytoplasmic localization whereas the unphosphorylated form locates almost exclusively in the nucleus (PubMed:24600006, PubMed:16495336). May be included in HIV-1 virions via its interaction with viral GAG polyprotein|
|Tissue Location||Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
BANF1 Antibody: Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BAF also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BAF will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BAF is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.
Furukawa K. LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction. J. Cell Sci. 1999; 112:2485-92.
Cai M, Huang Y, Ghirlando R, et al. Solution structure of the constant region of nuclear envelope protein LAP2 reveals two LEM-domain structures: one binds BAF and the other binds DNA. EMBO J. 2001; 20:4399-407.
Chen H and Engelman A. The barrier-to-autointegration protein is a host factor for HIV type 1 integration. Proc. Natl. Acad. Sci. USA 1998; 95:15270-4.
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