|Application ||WB, IHC-P, IF, E|
|Other Accession||AAH18435, 22450862|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||26253 Da|
|Application Notes||DRAM antibody can be used for detection of DRAM by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Other Names||DRAM Antibody: DRAM, DRAM, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator, damage-regulated autophagy modulator|
|Reconstitution & Storage||DRAM antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||DRAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73- mediated autophagy.|
|Cellular Location||Lysosome membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
DRAM Antibody: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.
Crighton D, Wilkinson S, O’Prey J, et al. DRAM, a p53-induced modulator of autophagy, is critical for apoptosis. Cell 2006; 126:121-34.
Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
Crighton D, Wilkinson S, and Ryan KM. DRAM links autophagy to p53 and programmed cell death. Autophagy 2007; 3:72-4.
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