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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   ARF-BP1 Antibody   

ARF-BP1 Antibody

  • WB - ARF-BP1 Antibody ASC10538
    Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 µg/mL.
  • ICC - ARF-BP1 Antibody ASC10538
    Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 µg/mL.
  • IF - ARF-BP1 Antibody ASC10538
    Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7Z6Z7
Other Accession NP_113584, 61676188
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 481891 Da
Application Notes ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 10075
Other Names ARF-BP1 Antibody: MULE, Ib772, LASU1, UREB1, HECTH9, URE-B1, ARF-BP1, HSPC272, KIAA0312, KIAA1578, E3 ubiquitin-protein ligase HUWE1, ARF-binding protein 1, HECT, UBA and WWE domain containing 1
Target/Specificity HUWE1;
Reconstitution & Storage ARF-BP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsARF-BP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HUWE1
Synonyms KIAA0312, KIAA1578, UREB1
Function E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of isoform 2 of PA2G4.
Cellular Location Cytoplasm. Nucleus. Note=Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells. {ECO:0000250|UniProtKB:Q7TMY8}
Tissue Location Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas
Research Areas
Citations (0)

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ARF-BP1 Antibody: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.


Gallagher SJ, Kefford RF and Rizos H. The ARF tumour suppressor. Int. J. Biochem. Cell Biol.2006; 38:1637-41.
Pomerantz J, Schreiber-Agus N, Leigeois NJ, et al. The Ink4a tumor suppressor gene product, p19ARF interacts with MDM2 and neutralizes MDM2’s inhibition of p53. Cell1998; 92:725-34.
Chen D, Kon N, Li M, et al. ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell2005; 121:1071-83.
Zhong Q, Gao W, Du F, et al. Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell2005; 121:1085-95.

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$ 330.00
Cat# ASC10538
Availability: 5-7days
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