|Application ||WB, IHC-P, IF, E|
|Other Accession||EAL24166, 51094921|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||39851 Da|
|Application Notes||STEAP1 antibody can be used for detection of STEAP1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. better|
|Target/Specificity||STEAP1; This STEAP1 antibody does not cross-react with other STEAP proteins.|
|Reconstitution & Storage||STEAP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||STEAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor (By similarity).|
|Cellular Location||Endosome membrane; Multi-pass membrane protein|
|Tissue Location||Ubiquitously expressed. Highly expressed in prostate tumors.|
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Provided below are standard protocols that you may find useful for product applications.
STEAP1 Antibody: The six-transmembrane epithelial antigen of prostate 1 (STEAP1) was the first member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. The normal function of STEAP is still uncertain; unlike other members of the STEAP family, STEAP1 does not promote iron or copper reduction or uptake and lacks the FNO-like reductase domain critical for activity. However, its expression is highly increased in multiple cancer cell lines, including prostate, bladder, colon, and ovarian cancers. Supporting this is evidence that STEAP1 peptides can be used to stimulate CD8+ T cells from healthy donors, enabling them to recognize STEAP1-positive human tumor cells, suggesting that STEAP1 may a potential target for cancer immunotherapy. At least three isoforms of STEAP1 are known to exist.
Hubert RS, Vivanco I, Chen E, et al. STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors. Proc. Natl. Acad. Sci. USA1999; 96:14523-8.
Ohgami RS, Campagna DR, McDonald A, et al. The Steap proteins are metalloreductases. Blood2006; 108:1388-94.
Ohgami RS, Campagna DR, Greer EL, et al. Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Nat. Genet.2005; 37:1264-9.
Alves PM, Faure O, Graff-Dubois S, et al. STEAP, a prostate tumor antigen, is a target of human CD8+ T cells. Cancer Immunol. Immunother.2006; 55:1515-23.
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