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Nhe-1 Antibody

  • WB - Nhe-1 Antibody ASC10605
    Western blot analysis of Nhe-1 in MOLT4 cell lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 µg/mL.
  • IHC - Nhe-1 Antibody ASC10605
    Immunohistochemical staining of human brain tissue using Nhe-1 antibody at 2.5 µg/mL.
  • IF - Nhe-1 Antibody ASC10605
    Immunofluorescence of Nhe-1 in Human Brain cells with Nhe-1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19634
Other Accession P19634, 127809
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 90763 Da
Application Notes Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 6548
Target/Specificity SLC9A1;
Reconstitution & Storage Nhe-1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsNhe-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC9A1
Synonyms APNH1, NHE1
Function Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Cellular Location Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Note=Colocalizes with CHP1 at the reticulum endoplasmic (By similarity). Colocalizes with CHP1 and CHP2 at the plasma membrane.
Tissue Location Kidney and intestine.
Research Areas
Citations (0)

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Nhe-1 Antibody: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.


Mendoza SA. The Na+-H+ antiport is a mediator of cell proliferation. Acta Paediatr. Scand.1987; 76:545-7.
Denker SP, Huang DC, Orlowski J, et al. Direct binding of the NA—H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation. Mol. Cell.2000; 6:1425-36.
Cingolani HE, Rebolledo OR, Portiansky EL, et al. Regression of hypertensive myocardial fibrosis by NA (+)/H(+) exchange inhibition. Hypertension2003; 41:373-7.

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$ 310.00
Cat# ASC10605
(40 western blots)
Availability: 5-7days
Bulk Size
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