|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_068589, 11141887|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||37775 Da|
|Application Notes||BRAL1 antibody can be used for the detection of BRAL1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||BRAL1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||BRAL1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates a firm binding of versican V2 to hyaluronic acid. May play a pivotal role in the formation of the hyaluronan- associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. Binds to hyaluronic acid (By similarity).|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Expressed only in adult brain.|
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Provided below are standard protocols that you may find useful for product applications.
BRAL1 Antibody: BRAL1 is a member a superfamily consisting of several highly homologous hyaluronan and proteoglycan binding link proteins. BRAL1 is predominantly expressed in brain tissue and spinal cord. Like other members in the link-module superfamily, BRAL1 contains an immunoglobulin-like fold and two proteoglycan tandem repeats (PTRs). Its mRNA expression pattern is similar to other lectican proteoglycans, suggesting that BRAL1 may act to stabilize the binding between the extracellular matrix molecule hyaluronan and brevican. Immunostaining of mouse brain showed BRAL1 expression at P20 in the white matter of the developing cerebellum and in myelinated fiber tracts in the adult brain, suggesting that expression starts when axonal myelination occurs.
Spicer AP, Joo A, and Bowling Jr. RA. A hyaluronan binding link protein gene family whose members are physically linked adjacent to chrondroitin sulfate proteoglycan core protein genes. J. Biol. Chem.2003; 278:21083-91.
Hirakawa S, Oohashi T, Su W-D, et al. The brain link protein-1 (BRAL1): cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain. Biochem. Biophys. Res. Comm.2000; 276:982-9.
Oohashi T, Hirakawa S, Bekku Y, et al. Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult mouse central nervous systems. Mol. Cell Neurosci.2002; 19:43-57.
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