|Application ||WB, E|
|Other Accession||EAW48578, 119568963|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||22496 Da|
|Application Notes||Akirin2 antibody can be used for detection of Akirin2 by Western blot at 0.5 µg/mL.|
|Reconstitution & Storage||Akirin2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Akirin2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for the innate immune response. Downstream effector of the Toll-like receptor (TLR), TNF and IL-1 beta signaling pathways leading to the production of IL-6. Forms a complex with YWHAB that acts to repress transcription of DUSP1 (By similarity).|
|Tissue Location||Widely expressed with the highest expression in peripheral blood leukocytes.|
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Provided below are standard protocols that you may find useful for product applications.
Akirin2 Antibody: The highly conserved, nuclear-localized Akirin1 and Akirin2 proteins critically regulate the transcription of NF-κB-dependent genes and are required for defense against Gram-negative bacteria in the immune deficiency and NF-κB pathways. Akirin1 is dispensable in the mouse, and neither knockout mice nor cells derived from them have obvious distinctive phenotypes. In contrast, Akirin2 is required for development in the mouse and knockout of both Akirin homologs in mice show that Akirin2 is required downstream of toll-like receptor (TLR), TNF-α and IL-1β signaling, and for the production of IL-6. Akirin2 is functionally closer to the single gene in Drosophila, as the homozygous null D. melanogaster Akirin mutants show a similar, mid-to-early embryonic death.
Goto A, Matsushita K, Gesellchen V, et al. Akirins are highly conserved nuclear proteins required for NF-kappaB-dependent gene expression in drosophila and mice. Nat. Immunol.2008; 9:97-104.
Beutler B and Moresco EM. Akirins versus infection. Nat. Immunol.2008; 9:7-9.
Sutterwala FS and Flavell RA. Immunology: cascade into clarity. Nature2008; 451:254-5.
Tanji T and Ip YT. Regulators of the Toll and Imd pathways in the Drosophila innate immune response. Trends Immunol.2005; 26:193-8.
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