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Rkhd1 Antibody

     
  • WB - Rkhd1 Antibody ASC10779
    Western blot analysis of Rkhd1 in MDA-MB-361 cell lysate with Rkhd1 antibody at (A) 1 µg/ml and (B) 2 µg/mL.
  • IHC - Rkhd1 Antibody ASC10779
    Immunohistochemistry of Rkhd1 in human small intestine tissue with Rkhd1 antibody at 2.5 µg/mL.
  • IF - Rkhd1 Antibody ASC10779
    Immunofluorescence of rkhd1 in human small intestine tissue with rkhd1 antibody at 20 µg/mL.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q86XN8
Other Accession Q86XN8, 134047829
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 64883 Da
Application Notes Rkhd1 antibody can be used for detection of Rkhd1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 399664
Target/Specificity MEX3D;
Reconstitution & Storage Rkhd1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsRkhd1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MEX3D
Synonyms KIAA2031, RKHD1, RNF193
Function RNA binding protein, may be involved in post- transcriptional regulatory mechanisms.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway.
Tissue Location Ubiquitously expressed in all the cell lines and tissues tested.
Citations (0)

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Background

Rkhd1 Antibody: Rkhd1, also known as TINO or MEX3D is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd1, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd1 binds to the AU-rich element in the 3'-untranslated region of Bcl-2 mRNA and is thought to be a negative regulator of Bcl-2 expression. Rkhd3 and Rkhd4, but not Rkhd1, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.

References

Donnini M, Lapucci A, Papucci L, et al. Identification of TINO: A new evolutionarily conserved Bcl-2 AU-rich element RNA-binding protein. J. Biol. Chem.2004; 279:20154-66.
Draper BW, Mello CC, Bowerman B, et al. MEX-3 is a KH domain protein that regulates blastomere identity in early C. elegansembryos. Cell1996; 87:205-16.
Liu J, Valencia-Sanchez MA, Hannon GJ, et al. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat. Cell Biol2005; 7:719-23.
Buchet-Poyau K, Courchet J, Le Hir H, et al. Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies. Nucleic Acids Res.2007; 35:1289-300.

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$ 320.00
Cat# ASC10779
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